Single Analytes

Most common markers for Systemic Lupus Erythematosus (SLE), Mixed Connective Tissue Diseases (MCTD), Sjögren's Syndrome (SS)

dsDNA | U1RNP | Sm | SS-A/Ro | SS-B/La | Rib-P | Mi-2  | Fibrillarin

Autoantibodies against

ssDNA NEW! | Histones (IgG/IgM) | Scl-70 | CENP | PM-Scl | Jo-1 

dsDNA

Products	Article No.	No. of tests
EliA dsDNA	14-5500-01	4x12 tests

Antigens

Deoxyribonucleic acid (DNA) as antigen may be either double-stranded (dsDNA) or single-stranded (ssDNA), but only dsDNA antibodies are specific markers. For use in anti-DNA assays, DNA from tissue, from eukaryotic cell, from bacteria or from bacteriophages is used. Circular plasmid DNA is a very suitable choice, because the risk to incorporate single strands is very low.

In the EliA assays the DNA is coated as recombinant plasmid double-stranded DNA

Sm

Products	Article No.	No. of tests
EliA SmDP -  not for sale in the U.S.	14-5624-01	4x12 tests
EliA Sm	14-5502-01	4x12 tests

Antigens

In their natural state, the small nuclear RNAs, also called U-RNAs (for "uracil rich RNAs"), exist as ribonucleoprotein particles (snRNP). The U snRNP U1, U2, U4, U5 and U6 all contain a group of proteins, the so-called Sm peptides, with the major targets being the B and D polypeptides. Because of cross-reactivity between the A, the C and the B/B' proteins, up to 60% of anti-U1 RNP sera may react with B/B'. Thus, only the presence of anti-D and/or the absence of anti-A and anti-C can be regarded as characteristic for an anti-Sm sera. Until now, all trials to produce an antigenic recombinant SmD protein with a good reactivity failed because of its very special structure. In 2004,

The EliA SmDP assay are coated with a synthetic SmD3 peptide. The EliA Sm Well is coated with a purified Sm antigen.

SS-A / Ro

Products	Article No.	No. of tests
EliA Ro52 - not for sale in the U.S.	14-5598-01	2x12 tests
EliA Ro60 - not for sale in the U.S.	14-5525-01	4x12 tests
EliA Ro	14-5503-01	4x12 tests

Antigens

The SS-A/Ro particle contains a hY RNA (human cytoplasmic RNA) and associated proteins: a 60 kDa and a 52 kDa protein. The 52 kDa protein is not directly bound to the hY RNA but to the 60 kDa protein. It seems that 52 kDA protein is sometimes associated to the SS-A/Ro particle and sometimes not. The Ro antigen is found in the cytoplasm but also in the nucleus. The role of the SS-A/Ro particle in the cell is not known yet.

EliA assays use human recombinant Ro 60 and Ro 52.

SS-B / La

Products	Article No.	No. of tests
EliA La	14-5504-01	4x12 tests

Antigens

The La/SS-B is a ubiquitously expressed phosphoprotein with 47 kDa, that associates with a variety of small RNAs including the hY RNA of the SS-A/Ro particle. La probably is a transcription termination factor for RNA polymerase III and is found in the cytoplasm as well as in the nucleus.

EliA assays make use of human, recombinant La.

Ribosomal P

Product	Article No.	No. of tests
EliA Rib-P - not for sale in the U.S.	14-5521-01	2 x 12 tests

Antigens

Systemic lupus erythematosus (SLE) is an autoimmune disorder characterized with multiple organ involvement resulting in disability and increased mortality. Antibodies against ribsosomal-P (anti Rib-P) react against acidic phosphorylated ribosomal proteins P0, P1, and P2 (with molecular mass of 38, 19, and 17 kDa, respectively) and are located on the S60 subunit of ribosomes. Anti Rib-P can be detected in approx. 15 to 20 % of patients with SLE. They appear highly specific for SLE; therefore, they can be used as diagnostic marker for the disease. Furthermore, association has been described with particular manifestations of lupus, especially with neuropsychiatric, renal, and hepatic involvements. However, results are conflicting regarding the existence of such associations, depending on different study set-up, different study population or different sensitivity of tests used for the detection of anti Rib-P.

The EliA Rib-P Wells are coated with human recombinant ribosomal P-proteins (P0, P1, P2).

Mi-2

Product	Article No.	No. of tests
EliA Mi-2 - not for sale in the U.S.	14-5604-01	2 x 12 tests

Antigens

There are two forms of Mi-2 proteins which show a high similarity (almost 70%): Mi-2α (208 kDa) and Mi-2β (218 kDa). Only Mi-2β seems to be the specific target for autoantibodies. Mi-2 seems to play a role in regulation of transcription. Mi-2β is a part of a nuclear complex named “nucleosome remodelling deacetylase (NuRD)”.

EliA Mi-2 Wells are coated with human recombinant Mi-2b protein produced in the baculovirus/insect cell system.

Fibrillarin

Product	Article No.	No. of tests
EliA Fibrillarin - not for sale in the U.S.	14-5606-01	2 x 12 tests

Antigen

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